Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions

photo of speaker Michael Hill, Ph.D.

Michael Hill, Ph.D.

Research Assistant, University of Buffalo

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The webinar demonstrates the use of SPR for measurement of cell adhesion interactions in biomedical applications.

Various biotechnology applications benefit from studies of cell-substrate interactions. A number of laboratories in the fields of biomedicine, biotechnology, and biophysics are keenly pursuing the nature of specific, receptor-ligand type interactions, and non-specific physical adhesive interactions.  

The Reichert SR7500DC dual channel system, a surface plasmon resonance (SPR) instrument with unmatched sensitivity and high-quality binding data for interaction analysis, is ideally suited for these applications. Researchers can use a large-tubing diameter that allows for the perfusion of cells, and the instrument’s open-design architecture accommodates ready manipulation of the SPR substrate.

Sponsored by Reichert Technologies Life Sciences, this free educational webinar, “Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions,” demonstrates the use of SPR for the measurement of cell adhesion interactions in varied biomedical applications, and presents two examples in which the Reichert SPR system has been used for studying cells.  The first example discusses human white blood cell (HL-60) adhesion/capture by the endothelial cell adhesion molecule P-selectin.  Several antibody- and recombinant protein-based controls are used to demonstrate the application of SPR for human vascular-biology research. The second example discusses binding interactions between endothelial cells and two different extracellular matrix proteins, Collagen 1 and Matrigel.

The speaker is research assistant Michael Hill. Michael who completed his PhD in biomedical engineering at the University of Buffalo this year, utilizing the SPR data in his thesis.  Michael’s advisor, Debanjan Sarkar, PhD, is in the laboratory of biomaterials and regenerative therapeutics.

The free webinar was hosted by LabRoots, and was presented on September 20, 2016.