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Reichert 2SPR

The best 2-channel SPR value you can buy

Choose the 2-channel surface plasmon resonance (SPR) system that lets you do more — and costs up to 30% less than the leading competitor. The Reichert2SPR System pushes the limits of detection and sensitivity, which is why it’s used by leading universities, academic facilities and biotech startups around the world for label-free biomolecular interaction analysis.

With Reichert, you get more, including:

  • High-quality, publication-ready data
  • The most sensitive, flexible and affordable 2-channel system available
  • The best performance-to-cost ratio for individual labs
  • A proven SPR leader since 1997

To see how Reichert can help advance your research and lead to new breakthroughs, contact us today to schedule a demo in your lab.

Request a Demo

Save up to 30% with Reichert — the affordable choice

Spend less money on equipment and maintenance — and more on your research — when you choose Reichert. In fact, the typical research lab can save $17,000 per year, per system, compared to the leading competitor. That’s $85,000 over the next five years — valuable funds that you can use for your staff and other critical research needs.

Lower Equipment Costs

Purchasing the Reichert2SPR System typically costs $25,000 less than the leading competitor.

Lower Operating Costs

Every SPR machine requires chips to couple proteins to. A typical lab can use 75–200 sensor chips each year, which is why you should always ask about the cost of chips when you’re considering an SPR system. At Reichert, our high-quality chips are often less than half the price of our competitors, so you can run more experiments without worrying about out-of-control costs.

No Costly Service Contract Required

Other SPR companies make you purchase a service contract, which can easily cost up to $150,000 over the course of five years. With the Reichert2SPR System, all of the typical parts that need maintenance are easy for you to access (and service) yourself. Of course, we do offer service contracts, if you would rather let us handle the regular maintenance.

Get High-quality, Publication-ready Data

The Reichert2SPR System offers significant advantages for researchers and scientists. Whether you’re working with simple solutions or more challenging assays, you can count on Reichert to offer:

  • High sensitivity — study protein interactions with low molecular weight (down to approximately 100Da) compounds; enhanced sensitivity also makes the Reichert2SPR System ideal for:
    • High quality kinetic analysis
    • Affinity measurements for weak or strong interactions (ranging from 1mM to 1pM)
    • Precise determination of thermodynamic parameters
    • Accurate concentration analysis
  • Industry-leading low drift (0.1 μRIU/min)
  • Extremely low noise (0.05 μRIU rms)
  • Faster baseline stability — the Reichert2SPR System stabilizes 2x-3x faster than the competition, due to our streamlined fluidics
  • High sample capacity (up to 768 samples per run)

Open System = More Control, More Flexibility, More Time For Research

Other SPR systems only allow for limited access to critical parts. But with Reichert, everything you need to access is right out in the open, which makes your life — and your research — much easier.

Easy To Change As Your Research Evolves

The Reichert2SPR System is designed to be as flexible as possible, from changeable tubes to an easily accessible flow cell. Wherever your research takes you in the future, you can be rest assured that Reichert will let you stay on track, without costly upgrades and changes.

5-minute Tubing Changes

With Reichert, you can change the tubing whenever you want in just minutes — without having to wait around for a service call. That’s because all Reichert SPR systems are designed to use low-cost, off-the-shelf HPLC fittings and tubing that are easily accessible to you and your staff. With Reichert, you can:

  • Change the tubing to different sizes
  • Accommodate a wide range of sample compositions (from pure soluble solutions to cell lysates), varying viscosities, and particulate matter
  • Run crude samples without having to purify them — saving you time and providing even greater flexibility — without risking costly delays and service calls; crude samples include:
    • Whole cells
    • Viruses
    • Lysates
    • Serum
    • Aggregates
  • Stop worrying about clogs — if a tube is damaged, blocked or clogged, you can replace it yourself in just a few minutes, using standard tubing

Easy-To-Clean Flow Cells

While some competitors charge nearly $10,000 to replace a flow cell cartridge, our flow cells are easy to clean (and, if necessary, change) yourself. We offer standard flow cells, as well as specialized flow cells that can be used simultaneously with other valuable analytical techniques, opening up new opportunities for investigation in SPR experiments.

A Wide Variety Of Standard and Specialized Chips

Our sensor chips perform equivalently to the leading competitor’s chips, at a significantly lower cost. Reichert offers all standard chips, including:

  • Carboxymethyl dextran
  • Planar polyethylene glycol/carboxyl
  • Nickel nitrilotriacetic acid
  • Hydrophobic planar alkyl
  • Streptavidin/NeutrAvidin
  • Protein A
  • Plain gold (if you want to add your own chemistry)

Specialized sensor chips are also available.

Interface With Other Modules For All-In-One Experimentation Options

Reichert’s open, component-based design lets you connect the Reichert2SPR System to other modules, and gain valuable new information in a single experiment.

Our specialized flow cells let you:

  • Combine Reichert SPR with:
    • Electrochemistry
    • Fluorescence
    • Photochemistry
  • Couple Reichert SPR with:
    • Mass spectrometry
    • Liquid chromatography

Choose An Automatic Or Semi-Automatic System

The Reichert2SPR System is a two-channel system, which means you can run one experiment at a time (the other channel is for your reference data); we also offer a four-channel system, which costs more but lets you perform 3x as many experiments).

Our two-channel system is available in two configurations:

Automatic — For Up To 24 Hours Of Unattended Analysis Time

  • Autosampler automatically injects your samples while you do other work or leave the lab
  • Saves time and produces faster results
  • Maximizes the performance and throughput

Semi-Automatic — A More Cost-effective Choice

  • You or your staff manually inject samples (ideal when working with a limited sample set)
  • Perfect for training students and staff on injection techniques
  • Upgradable at any time

Need more channels? See our 4-channel instrument.

User-friendly Software Lets You Get Work Done Faster

Streamline your workflow with our powerful yet easy-to-use software. The simple drag-and-drop interface lets you quickly develop and execute experiments, including couple and capture experiments.

The Autolink software controls and analysis package features:

  • 21 CFR part 11 controls
  • Flexible, drag-and-drop experiment setup
  • Streamlined data acquisition and analysis
  • Flexible data analysis with multiple binding models
  • Access controls to ensure data integrity
  • Configurable authorization levels for creating and running experiments, options and data export

Reichert is a name you can trust. We’ve been working with SPR technology since 1997, selling to some of the top names in academics and private industry. We’ve been in business for more than 140 years. And we’re backed by a billion-dollar company, AMETEK, Inc.

Personal Service And Attention, Whenever You Need It

At Reichert, we’re here for you — just as we have been for more than 20 years — with the expert service and support you expect from an industry leader.

  • Product demo in your lab: We’ll bring a machine to your lab, set it up, and show you how to run your samples so you can see the results for yourself. Request a demonstration.
  • In-person set-up and training: When you purchase a Reichert system, we’ll come to your lab to set everything up for you and help train your staff.
  • Ongoing support and troubleshooting: If you ever have a question or problem, just give us a call or send an email. We’ll help you address any issues, at any time.

Surface plasmon resonance (SPR) is a technique that lets you investigate biomolecular interactions (including, but not limited to, protein-protein binding and protein-small molecule binding) in real-time. Specifically, it provides real-time data regarding the binding kinetics and affinity for a plethora of molecular interactions. With SPR, you can determine if there is an interaction with your protein or other target molecule — and, if so — answer questions about the interaction including:

  • How fast do the molecules associate and dissociate?
  • How strong is the interaction (affinity)?
  • How specific is it?

If you have questions about whether or not SPR is right for your lab, please contact us.

For Early-stage Life Sciences And Material Science Research

SPR is typically used in academic and industry settings to advance research in a range of areas, including:

  • Antibody development
  • Drug development and discovery
  • Concentration analysis
  • Gene regulation
  • Immunogenicity
  • Nanoparticles/Nanomaterials
  • Pharmaceutical research
  • Studying protein structure and function determination
  • Systems biology
  • Thermodynamics analysis (ΔH & ΔS)

Whether you’re studying Alzheimer’s disease, developing new vaccines or focused on other areas of protein research, SPR can help you quickly determine whether proteins interact, and which ones are worth studying more.

Determine The Binding Properties Of Biomolecular Interactions

SPR allows you to study a variety of interactions, including those involving:

  • Proteins
  • Nucleic acids
  • Lipids
  • Carbohydrates
  • Small molecules
  • Bacteria
  • Polymers

With Reichert’s accessible tubing, you can also perform SPR on crude samples, including:

  • Whole cells
  • Viruses
  • Lysates
  • Serum

Label-free Technique Saves You Time

With SPR, you work with native proteins, which means you don’t have to attach another molecule to one of your proteins. Instead, you’ll use our SPR device to immobilize one of your proteins on a small “chip,” and then flow your other protein or molecule over the chip to see the response and study the interaction.

SPR: More Data, Greater Accuracy Than Other Techniques

With SPR, you get a large volume of highly accurate information about protein interactions, including the strength of the interactions (affinities) and the binding kinetics — how fast the molecules are binding (associating) and dissociating. Other techniques don’t provide as much data and aren’t as accurate.

Technical Information

Transducer Principle Kretschmann prism, multiple angles from fixed light input
Measurement Channels – Two (either parallel or series fluid connection)
Sample Loading Autosampler or semi-automatic injector, standard HLPC tubing and connectors, external syringe pump
Sample Capacity Any combination of up to 2 trays can be used. Choose: 12 (10 mL) or 48 (2 mL) vials; or 96-well (high or low) or 384-well plates
Sample Storage 4°C or ambient temperature
Flow Cell Volume (per channel) – 0.18 µL Flow Cell Surface Area (per channel) – 4.5 mm² (reference value)
Aspect Ratio > 25 (width/height)
Fluid Contact Materials Teflon™, Acetal Copolymer, PEEK™, Kalrez™, ETFE (Tefzal™)
Sample Volume 20-4500 µL (depends on installed loop volume)
System Fluid Volume 28 µL (typically) (0.01" I.D. tubing) or 7.5 µL (0.005" I.D. tubing)
Temperature Range 10°C below ambient to 70°C

Measurement Sensitivity

Refractive Index Resolution < 10-7 RIU (< 0.1 µRIU)
Refractive Index Range 1.33 to 1.40 (@780nm)
Analyte Concentration Range 1 mM to 1 pM
Minimum Molecular Weight Detection < 100 Daltons
Baseline Noise 0.1 µRIU peak-to-peak, 0.05 µRIU RMS, @ 25 mL/min
Baseline Drift < 0.1 µRIU/min

Typical Kinetic and Equilibrium Constant Ranges

Association Rate Constant 103 to 107 M-1 s-1
Dissociation Rate Constant 10-1 to 10-5 s-1
Equilibrium Dissociation Constant 0.1 mM to 1 pM
Electrical AC Power Supply, standard international voltage range w/universal adapter from 100 to 240 V; 50 to 60 Hz
Regulatory Compliance with the applicable sections of the European EMC Directive and IEC safety requirements for laboratory electrical equipment for measurement and control
Product Safety Compliance with IEC 61010-1 (Low Voltage Directive) under a Category classification EMC and Safety: CE mark certification (Class A, Type II)

The Reichert2SPR - Reichert’s SR7500DC system is the smart choice for scientists and lab managers. Reichert surface plasmon resonance instruments provide a highly sensitive and flexible platform to meet the demands of a variety of applications. In comparison with the Biacore X100, Reichert’s maintenance costs, autosampler capacity, and sensitivity make it the obvious choice.

  Reichert2SPR Biacore X100
Number of Channels 2 2
Injection of Lysates, Cells, Particulates Yes No
DIY Fluidics Yes No
Autosampler Capacity Maximum 768 samples 15 samples
Baseline Drift 0.1 RU/min < 0.3 RU/min
Baseline Noise 0.05 RU(RMS) < 0.1 RU(RMS)
Sensor Chip Cost $84 for dextran chip $150 for dextran chip
Specialized Flow Cells Electrochemistry, Photochemistry, Fluorescence, Mass Spectrometry None
Sample Volume 20 – 4500 µL 20 – 30 µL (required)
Temperature Range 10°C below ambient to 70°C 4°C to 45°C (maximum 10°C below ambient)
Sample Compartment Temperature 4°C or Ambient None
Flow Rate Range 0.1 – 3000 µL/min 1 – 100 µL/min
Minimum Molecular Weight Detection < 100 Da 100 Da

See for yourself how the Reichert4SPR System can help you make new discoveries. Contact us today, to set up a hands-on demonstration in your lab.

Request a Demo

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