Planar Polyethylene Glycol/Carboxyl Sensor Chip

Price: varies

SKU / Part #: 13206061

This surface consists of a mixed, self-assembled monolayer of alkanethiolates generated from the combination of polyethylene glycol-terminated alkanethiol (90%) and COOH-terminated alkanethiol (10%). The terminal polyethylene glycol chains minimize non-specific binding while the COOH groups provide a functional attachment site for immobilizing/capturing a molecule of interest. Amine coupling, utilizing EDC/NHS chemistry, is the most common ligand immobilization approach but thiol, aldehyde, and maleimide coupling are also possible with this surface using the appropriate cross-linking chemistry.

Here are the protocols for this sensor chip:

Capture of His Tagged Protein over Anti-His
Method for Amine Coupling
Protocol for Thiol Coupling via either Surface or Ligand Coupling
Protocol for Steptavindin NeutrAvindin Immobilization

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