While there are many biosensing instruments available, SPR technology makes observing binding behavior on a molecular level easier and more accurate than ever before. Here are just a few distinct advantages associated with the use of surface plasmon resonance:
1. LABEL-FREE DETECTION
Differences in refractive index are detected using SPR so no label is needed for detection. You can observe, in real-time, biomolecular interactions between a variety of proteins, DNA/RNA and small molecules.
2. SMALL SAMPLE SIZES
SPR requires a minimal amount of sample to run experiments. Scientists and healthcare professionals can use less of expensive materials, thus saving money and making surface plasmon resonance more affordable and accessible.
3. REUSABLE SENSOR CHIPS
Another cost effective advantage associated with SPR is the ability to reuse sensor chips. Sensor chips directly influence the quality of the data and serve as a vital component of biosensing. With so much depending on the quality and performance of the sensor chip, it is even more impressive that these chips can be reused.
4. ABILITY TO HANDLE COMPLEX SAMPLES
Even in situations where only crude samples are available for testing, surface plasmon resonance can be used. SPR has been used for testing samples in a variety of complicated matrices including for serum analysis.
5. ABILITY TO REPLICATE MEASUREMENTS
Replicate injections of the same concentration of sample throughout a run can be done in a repeatable manner. Between day calculations of rate constants are also found to be comparable, increasing confidence in the accuracy of results.
6. REAL-TIME MONITORING
Essentially, the use of SPR technology provides a simple and relatively affordable way to observe how different biomolecules interact in real time. This has tremendous applications in the pharmaceutical and medical industries as well as the fields of genetics and kinetics. New applications with materials such as nanoparticles are also a burgeoning field of interest.