Surface Plasmon Resonance (SPR) in combination with phage display can be used for antibody production. This combination can reduce the number of steps required to select out antibodies and can yield additional data, including kinetics and affinity information. This combination of techniques is an excellent tool; one example of its applicability is in the field of proteomics research. SPR requires smaller sample sizes in comparison with traditional ELISA. As with recombinant methods of antibody development, a phage display library does not require the use of laboratory animals; thereby saving weeks of research time.

Real-time analysis and label-free detection using SPR helps scientists in the purification of high affinity antibodies. SPR affords lab-on-a-chip capabilities to scientists using phage display for highly efficient antibody production. The process of phage injection over an SPR sensor chip allows for real-time and label free detection of antibodies as they bind to the target antigen. Bound antibodies can then be easily eluted and removed for further analysis or selection. Using SPR in conjunction with phage display provides a more automated way to vary phage conditions. This can lead to greater throughput; sometimes yielding as much as a six-fold increase (when compared with more traditional approaches). Scientists using this technique have maximized the efficiency and speed of antibody production while conducting research with Reichert SPR instruments.

Phage display technique can quickly provide an abundance of probes and analytes for chip analysis. It offers an ideal method to quickly analyze and select antibodies when used in combination with SPR technology. Using SPR, scientists can separate out high affinity antibodies, then quickly isolate and efficiently characterize them with other techniques as needed. Efficiency, optimal control and reproducibiity are major reasons why SPR, in combination with phage displays, can provide a competitive advantage in the expanding field of antibody therapeutics for autoimmune diseases, transplantation and cancer.

Sources

http://www.biosciencetechnology.com/article/2016/05/using-surface-plasmon-resonance-phage-display-libraries

http://www.sciencedirect.com/science/article/pii/S0925400508007909

http://www.prweb.com/releases/2016/04/prweb13357564.htm