Antibodies: High-Capacity

Sensor Chips

Plain Gold Surface

Sensor Chip

  • Surface: bare gold
  • Study surface formation and adsorption in real-time
  • Add your own surface coatings
  • Can also be used for SPR Electrochemistry experiments

Carboxymethyl Dextran Hydrogel Surface

Sensor Chip

  • Surface: Carboxymethyl Dextran Hydrogel
  • A stable surface resistant to non-specific binding
  • Use for large/medium sized molecules and their interactions with low molecular weight molecules

Dextran Protein A

Sensor Chip

  • Surface: Protein A
  • Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)
  • Use for capturing Fc region from IgG of several species including human and rabbit
  • High capacity chip used for interactions between small molecules and peptides with large molecules or interactions between large molecules.

Polycarboxylate Hydrogel

Sensor Chip

  • Surface: Polycarboxylate Hydrogel
  • Provides another choice for high capacity coupling when studying protein-small molecule interactions or when dextran is not ideal
  • Must purchase a 0.3mm flow cell
  • Use for large/medium sized molecules and low molecular weight molecules

Streptavidin Covalently Immobilized on a Carboxymethyl Dextran Surface

Sensor Chip

  • Surface: Streptavidin
  • Minimal biotinylation of the ligand and subsequent capture results in an oriented arrangement of ligand molecules on the surface
  • Use for high affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

High Capacity Nickel Nitrilotriacetic Acid Surface 200M

Sensor Chip

  • Surface: Nickel Nitrilotriacetic Acid
  • High capacity capture works well even for protein-small molecule interactions
  • Must purchase a 0.3 mm flow cell
  • Use for capture of Poly His-tagged Molecules
  • Results in an oriented capture
  • Chip can be reused as long as capture is reversible

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