Antibodies: High-Capacity
Sensor Chips
Plain Gold Surface
Sensor Chip
- Surface: bare gold
- Study surface formation and adsorption in real-time
- Add your own surface coatings
- Can also be used for SPR Electrochemistry experiments
Carboxymethyl Dextran Hydrogel Surface
Sensor Chip
- Surface: Carboxymethyl Dextran Hydrogel
- A stable surface resistant to non-specific binding
- Use for large/medium sized molecules and their interactions with low molecular weight molecules
Dextran Protein A
Sensor Chip
- Surface: Protein A
- Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)
- Use for capturing Fc region from IgG of several species including human and rabbit
- High capacity chip used for interactions between small molecules and peptides with large molecules or interactions between large molecules.
Polycarboxylate Hydrogel
Sensor Chip
- Surface: Polycarboxylate Hydrogel
- Provides another choice for high capacity coupling when studying protein-small molecule interactions or when dextran is not ideal
- Must purchase a 0.3mm flow cell
- Use for large/medium sized molecules and low molecular weight molecules
Streptavidin Covalently Immobilized on a Carboxymethyl Dextran Surface
Sensor Chip
- Surface: Streptavidin
- Minimal biotinylation of the ligand and subsequent capture results in an oriented arrangement of ligand molecules on the surface
- Use for high affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids
High Capacity Nickel Nitrilotriacetic Acid Surface 200M
Sensor Chip
- Surface: Nickel Nitrilotriacetic Acid
- High capacity capture works well even for protein-small molecule interactions
- Must purchase a 0.3 mm flow cell
- Use for capture of Poly His-tagged Molecules
- Results in an oriented capture
- Chip can be reused as long as capture is reversible