Whole Cell and Crude Samples

How do you avoid clogs while studying whole cell and crude samples? Choose Reichert. With Reichert Surface Plasmon Resonance (SPR) systems, you can keep different sizes of tubing on-hand in your lab and easily change the tubing yourself in just minutes. Use larger tubing to essentially eliminate clogs, with no loss of performance. If your tubing does clog, it’s easy for nearly anyone in your lab to change it themselves — without waiting for a costly, time-consuming repair technician.

• Reichert’s open architecture is much more rugged and versatile compared to systems that employ delicate (and costly) microfluidics cartridges
• Choose from a range of tubing diameters — up to 254 µm (0.01” ID) for the inlet — to prevent clogs
• Study phage display, whole cells and serum with confidence

With Reichert, it’s fast, easy and cost-effective to accommodate nearly any sample.

Application Notes

Phage-antibody selection

• Application Note 12
  • Preliminary study on the topic

Serum IgG antibody binding to recombinant Ebola virus glycoprotein

• Application Note 13
  • Dissociation rate comparison

SPR for cell studies

• Application Note 16: Exploring the nature of endothelial cell (EC) adhesive interactions
• Application Note 17: Measuring white blood cells (leukocyte) recruitment onto substrates bearing vascular adhesion molecules
  • Two different examples of combining SPR with cell-based research carried out by a researcher at the University at Buffalo (UB)

Publications

Michael J. Hill and Debanjan Sarkar, “Comparative surface energetic study of Matrigel® and collagen I interactions with endothelial cells,” Colloids and Surfaces B: Biointerfaces, 155, 2017, pp. 71–82.

Sadagopan Krishnan; Vigneshwaran Mani; Dhanuka Wasalathanthri; Challa V. Kumar; James F. Rusling. “Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels,” Angew. Chem. Int. Ed. 2011, 50, 1175 -1178.