Protein-Low Molecular Weight Applications

With Reichert, you can analyze interactions between proteins and small molecules — down to compounds with molecular weights of 100 Da or less. Reichert’s two-channel and four-channel Surface Plasmon Resonance (SPR) systems give you low noise, low drift, streamlined workflows for multi-sample runs, and a built-in DMSO correction feature for compounds that aren’t water-soluble. These are just a few of the reasons why leading labs around the world choose Reichert for SPR.

For a Standard Amine Coupling Experiment

  • The protein will be the ligand/target
  • The small molecule will be the sample that is flowed over
  • We recommend this setup because most small molecules do not have free primary amines that can be used for attachment via amine coupling

For a Typical 1:1 Binding Experiment

  • The calculated maximum response is estimated as the ratio of the sample molecular weight divided by the ligand/target molecular weight times the amount of ligand/target coupled
  • This means that the ligand/target surface density needs to be high in order to see responses
  • Therefore, this type of experiment is run using either dextran or another hydrogel surface

Application Notes

Small Molecule Assay: 4-Carboxybenzenesulfonamide (201 Da) Binding to Carbonic Anhydrase II

95 Dalton and 201 Dalton Analyte SPR Analysis Using a Reichert SR7500DC and Carboxymethyl Dextran Slides

  • Application Note 7
    • Features two different small molecule examples which are typical for SPR

DNA Binding to Zinc Complexes


Jamie A. Moroco, John Jeff Alvarado,  Ryan P. Staudt,  Haibin Shic, Thomas E. Wales, Thomas E. Smithgall, John R. Engen, “Remodeling of HIV-1 Nef Structure by Src-Family KinaseBinding,” Journal of Molecular Biology, Available online 16 December 2017, ISSN 0022-2836,

Chuanbo Liu, Zuojia Liu and Jin Wang, “Uncovering the molecular and physiological processes of anticancer leads binding human serum albumin: A physical insight into drug efficacy,” PLOS ONE April 20, 2017,

Christian Brengel, Andreas Thomann, Alexander Schifrin, Giuseppe Allegretta, Ahmed A. M. Kamal, Jçrg Haupenthal, Isabell Schnorr,[ Sang Hyun Cho, Scott G. Franzblau, Martin Empting, Jens Eberhard, and Rolf W. Hartmann, “Biophysical Screening of a Focused Library for the Discovery of CYP121 Inhibitors as Novel Antimycobacterials,” ChemMedChem, 2017, 12, 1616 – 1626, DOI: 10.1002/cmdc.201700363.

Leading Labs Choose Reichert

  • Performance and sensitivity to run routine experiments extremely well
  • Added capability to perform specialized applications