Method Development Notes

Review guides, read tips, and get help for developing experimental methods. At Reichert, we’re here to help you every step of the way — from choosing the right Surface Plasmon Resonance (SPR) instrument for your lab, to setting up successful experiments and helping you get the most accurate results. We encourage you to read our method development notes, and contact us if you have additional questions.

Slides (Sensor Chips)

See “Choosing the Right Sensor Chip” (blog post) and the Sensor Chips section of this website for protocols.

• Dextran
• Planar
• Streptavidin/NeutrAvidin
• Nickel-NTA
• Planar Protein A

Experiments

• Capture
• Couple

See “Immobilization Strategies” (blog post) for more information to compare and contrast these experiments.

Regeneration Solutions

• Acidic
• Basic
• Ionic

See “Overcoming Regeneration Problems” (blog post) for more information.

Other Issues to Consider

• Non-specific binding: See “Reducing Non-Specific Binding” (blog post)
• Mass transport interference: See “Tips for Users Series - #1” (blog post)