Combining SPR with Other Techniques

Reichert systems have the open architecture, full accessibility and easy-to-change tubing which allow you to combine Surface Plasmon Resonance (SPR) with a wide variety of analytical techniques. If you want to get better, faster results, use Reichert to combine:

• Electrochemistry + SPR
• Mass spectrometry + SPR
• Photochemistry + SPR
• Additional analytical techniques + SPR

For the versatility you need to advance your research as efficiently as possible, choose Reichert.

More Flexibility for Your Lab

Here are just a few of the ways you can use SPR with other techniques:

SPR + Electrochemistry

• Monitor polymer formation
• Study compounds that selectively bind based on redox state

SPR + Mass Spectrometry

• Identify what has bound to the ligand or target from a mixture
• Use for epitope analysis

SPR + Photochemistry

• Study samples that fluoresce to bind or unbind

Application Notes

Combining Electrochemistry with SPR

• Application Note 5
• Follows the polymerization of aniline

Combining Mass Spectrometry with SPR for Epitope Analysis

• Each application note provides an example of what region on an antigen a specific antibody binds to
  • Application Note 14: AΒ Specific Antibodies
  • Application Note 15: Horse Heart Myoglobin

Recent Publications

Mass Spectrometry

Zdenek Kukacka, Marius Iurascu, Loredana Lupu, Hendrik Rusche, Mary Murphy, Lorenzo Altamore, Fabio Borri, Stefan Maeser, Anna Maria Papini, Julia Hennermann, and Michael Przybylski, “Antibody Epitope of human α-Galactosidase A revealed by affinity-mass spectrometry: A basis for reversing immunoreactivity in enzyme replacement therapy of Fabry’s Disease,” ChemMedChem (2018), 10.1002/cmdc.201800094

Claudia Henn; Stefan Boettcher; Anke Steinbach; Rolf W. Hartmann. “Catalytic enzyme activity on a biosensor chip: Combination of surface plasmon resonance and mass spectrometry.” Anal. Biochem. 2012, 428, 28-30.

Nico Dankbar, Erk Gedig and Simone König. “A Surface Plamson Resonance/Mass Spectrometry Interface for Maximum Sensitivity.” Biomacromolecular Mass Spectrometry 2010, 2, 77-80

Fluorescence

Catalina Sandoval-Altamirano, Susana A.Sanchez,. Nancy Ferreyra and German Gunther, “Understanding the interaction of concanavalin a with mannosyl glycoliposomes: A surface plasmon resonance and fluorescence study,” Colloids and Surfaces B: Biointerfaces, 2017, 158, pp 539-546, doi.org/10.1016/j.colsurfb.2017.07.026.