Reichert SPR Small Volume InjectionsAppilcation Note #4
Small Volume Injections with SR8500 Syringe Pump
The Reichert SR8500 syringe pump includes a feature for performing small volume injections referred to as recycle mode. The recycle mode is ideal when a research project is sample volume limited. This unique feature is typically used either for immobilization of ligand or to obtain equilibrium or kinetic data from analyte injections when only minimal sample volume is available for injection (1 - 50 μL).
A small volume of sample can be aspirated by the pump and then recycled to conserve on sample. To accomplish this, a short piece of tubing is connected to the flow cell outlet (4” long x 1/16” OD x 0.005” ID, volume = 1.3 μL). This tubing is submerged directly into the sample container (e.g., a microcentrifuge tube) and the sample is aspirated into the flow cell at a particular rate. The sample is then pumped back and forth in the flow cell at a defined recycle rate. The range of available volumes and rates is as follows:
- Aspirate Volumes: 5, 10, 15, 20, 30, 40, 50 μL
- Aspirate Rates:1, 2, 5, 10 μL/min.
- Recycle Volumes1-10 μL
- Recycle Rates25, 50, 100, 200, 500 to 6000 in 500 increments (μL/min.)