About

Why Surface Plasmon Resonance (SPR)?

SPR gives you more data — more accurately — compared to other analytical techniques, including Isothermal Titration Calorimetry (ITC), Biolayer Interferometry (BLI) and Microscale Thermophoresis (MST). With SPR, you can determine the rates and affinity of interactions between biomolecules, and answer multiple questions using a single instrument.

SPR is the gold standard for analyzing biomolecules, providing:

  • High quality kinetics (association and dissociation constants, plus equilibrium)
  • Real-time data acquisition
  • Label-free analysis
  • Faster, automated experiments
  • Lower sample consumption
  • The only method accepted by regulatory authorities

High quality kinetics are critical for determining whether a therapeutic candidate should move forward in the discovery process, and for providing quantitative data for publication. Given the increasing need for highly accurate quantitative techniques, SPR is an invaluable tool for biological and pharmaceutical R&D, production and QC. In fact, compared to ITC, BLI and MST, SPR is the only technique that fulfills the requirements of regulatory authorities including the FDA, EMA and ICH.

Compare SPR to other techniques, and you’ll quickly see why leading labs choose SPR.

Applications for SPR

SPR’s flexibility lets you study biomolecular interactions in a wide variety of analytes, from small molecules in drug discovery to peptides, proteins, DNA, viruses and even whole cells. SPR allows for:

  • Simple yes/no binding
  • Equilibrium studies
  • Complex kinetic analyses
  • Thermodynamic analysis
  • Concentration determination

With Reichert’s robust and flexible systems, you can even analyze crude samples and undiluted serum, and use SPR in tandem with liquid chromatography systems and/or mass spectrometers.

SPR: More Data, More Flexibility

SPR is the most versatile and information-rich analytical method, which is why SPR is the instrument of choice for universities and research organizations around the world.