Proteins: Low-Capacity
Sensor Chips
Plain Gold Surface
Sensor Chip
- Surface: bare gold
- Study surface formation and adsorption in real-time
- Add your own surface coatings
- Can also be used for SPR Electrochemistry experiments
Planar Protein A
Sensor Chip
- Surface: Protein A
- Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)
- Use for capturing Fc region from IgG of several species including human and rabbit
- Low capacity chip used for interactions between large molecules
Nickel Nitrilotriacetic Acid
Sensor Chip
- Surface: Nickel Nitrilotriacetic Acid
- High capacity capture works well even for protein-small molecule interactions
- Must purchase a 0.3 mm flow cell
- Use for capture of Poly His-tagged Molecules
- Results in an oriented capture
- Chip can be reused as long as capture is reversible
NeutrAvidin Covalently Immobilized on a Planar mSAM Surface
Sensor Chip
- Surface: NeutrAvidin
- Minimal biotinylation of the ligand and subsequent capture results in a more oriented arrangement of ligand molecules on the surface
- Use for capture of biotinylated molecules such as proteins, peptides, and nucleic acids