Why Surface Plasmon Resonance (SPR)?

If you need high quality kinetics, real time data acquisition, label free analysis, and automated experiments, Surface Plasmon Resonance technology is the gold standard.  SPR also provides the ability to obtain equilibrium binding constants, thermodynamic analysis, and concentration determination. 

These capabilities make Surface Plasmon Resonance (SPR) an increasingly popular technique for determining the rates and affinity of interactions between biomolecules. The technique has found widespread use in applications involving simple yes/no binding, equilibrium studies and complex kinetic analyses. The range of applications includes (to name a few): protein-protein, protein-DNA, DNA-DNA and protein-small molecule drug binding. 

Advantages of SPR compared to other techniques are:

  • Faster experiments
  • Lower sample consumption
  • Higher quality kinetics - association & dissociation constants, in addition to equilibrium

High quality kinetics are critical for determining whether a therapeutic candidate should move forward in the application process and for providing quantitative data for publication.  Given the increasing need for quantitative techniques and ability to answer multiple questions from a single instrument, Surface Plasmon Resonance (SPR) is a necessity for today's biomolecular laboratories.