SPR System Software for Reichert SPR Instruments

New Reichert4SPR System Software

Reichert sets the standard for easy-to-use SPR with new software for the Reichert4SPR - 4-Channel SPR Instrument. Redesigned with the scientist in mind, the new software is intuitive, easy-to-use and powerful.


Listening to feedback from scientists who were frustrated with other SPR systems that made them feel like a computer programmer, Reichert’s new software is designed around the scientist and their needs. This new instrument control software guides the user through the natural workflow of an experiment – from immobilization to method development and through scale up of large screening runs. No training is required and the concept of “programming” a run does not exist – allowing the focus to be on the science, while the software runs the instrument. The new software significantly reduces experiment set-up time and manual entry for the scientist. The enhanced Reichert4SPR is intuitive and only asks the scientist about relevant experimental details.

An important benefit of the new software is the organization of projects and experiments. The system does it for the user. Want to build on a previous experiment? Call it up, modify only the parameters they want to change and begin.  The software is perfect for core facilities and pharma/biotech companies. With a welcoming, clean look and feel, the software is not intimidating and easily walks you through the set up and execution of your experiment.

Consistent with Reichert’s philosophy, the software is ready to support GxP environments and is 21 CFR Part 11 compliant. No additional software or modules are required. Existing Reichert4SPR systems are compatible with the new software and the upgrade is available.  With the new software, Reichert takes ease of use to an entirely new level, while maintaining the extremely high sensitivity and flexibility scientists require for the most demanding and creative experiments.

Reichert2SPR System Software

Reichert's powerful software platform for the SR7500DC 2-Channel SPR Instrument, provides integrated data acquisition, automated sample handling, and data analysis:

  • Integrated sophisticated control of the Reichert SPR System 
  • Flexible, intuitive, step-by-step control of the Surface Plasmon Resonance system.
  • Drag and drop methods to set up multi-sample run tables.
  • Manual control of each component individually without constraints – the ultimate flexibility for real-time method development

Reichert's SPR software is validated with 21 CFR part 11 technical controls

Protecting the security and integrity of electronic records (ER) is essential for compliance. This includes ensuring the reliability and trustworthiness of ER used to support critical decisions. Features in Reichert’s SPR software include:

  • Data security and integrity – access control along with file encrypted checksums
  • User authorization levels – administrator, factory, and user levels set access rights to software functions
  • Record tracking – experiment details, events, and user identities are logged in a date- and timed-stamped secure file
  • Data can be exported both manually and automatically in a variety of formats, including tab delineated text files and Scrubber® data analysis files

The software has been developed in accordance with an accepted development model to ensure adequate validation.

Experimental Method Development

https://asoft8280.accrisoft.com/reichert/clientuploads/directory/B Program Run Table.jpg
Developing an experimental method with drag and drop functionality is easy. To begin, a standard method can be dragged and dropped from the “Available Methods” section (center) to the run table window. The method can be quickly modified as necessary by the researcher. User defined methods can be saved for reuse in future experiments. In the example sequence, step 1 is a pump refill and step 2 is a 1-minute wait for baseline stabilization. The third step is a 2 minute 100µL sample injection from vial 1A followed by a 5 minute dissociation period. The final steps are a 50µL injection of a regeneration solution followed by a 1 minute dissociation. The checked “Rinse” box in an injection method washes the sample loop and needle with buffer prior to the next sample injection. Multiple injections are easily programmed by simply dragging and dropping a “Loop” command at the beginning and end of the methods that are to be run several times. The number of loops is set in a user-definable box in the loop end command. The vial position will automatically increment.  This functionality easily facilitates scale-up of experiments in minutes, to take advantage of Reichert's high capacity autosampler - including injections from 96 and 384 well plates or 48 or 12 vial sample trays.

Data acquisition window
https://asoft8280.accrisoft.com/reichert/clientuploads/directory/A-Data Acquistion-Real Time Visualization.jpg

Data from the individual channels and difference can be viewed together or separately in real-time. Both the response (Y axis) and time (X axis) axis may be auto-scaled or set to user-defined limits. Individual cycles can be selected to quickly zoom in on an area of interest. The data window can also be expanded over a defined region to allow close examination of the SPR response curves. In addition, this window continuously displays the status of each system component, read time interval, flow rate, temperature, file name and injection/dissociation markers.

Post Process Window

https://asoft8280.accrisoft.com/reichert/clientuploads/directory/C-Post Process.jpg
SPR sensorgrams are automatically extracted and aligned to the same zero start time and zero response. The aligned sensorgrams are then sent to the analysis package. In addition to Reichert's integrated package, data can be exported for use in other widely available packages, such as Scrubber.

Analysis

Aligned sensorgrams are automatically transferred to the integrated data analysis package in one click. Once in the analysis module, users can fine-tune the alignment, perform background and blank subtractions, and adjust concentrations, if needed. Creating kinetic fits is a straight forward task, and results are clearly displayed. There are a number of kinetics models to choose from to ensure appropriate characterization of the experimental data. Data can be fit to a 1:1 binding model (with or without mass transport or depletion), or three different 2-site models (2:1 with or without conformational change, or 1:2). You can also fit Dissociation data only. Figure 1 shows results for data fit to a 1:1 binding model.

Binding Model
Figure 1: Data fit to a 1:1 Binding Model

Additionally, when equilibrium responses are obtained, affinity analyses can be completed to create figures that are publication- ready (see Figure 2).

TraceDrawer can be used for Affinity plots
Figure 2: Sample Affinity Plot

Summaries of ka(association rate constant) an kd(dissociation rate constant) can be presented as on/off plots by clicking the On/off rate map button in the summary tool. Each curve is presented as a coordinate in the ka/kd-space, which enables a fast overview of many compounds.

clientuploads/blog_images/TraceDrawer Figure 3.jpg
Figure 3: Easily create publication-ready figures, such as this summary of screening of five different compounds over the same surface.

Reichert's Surface Plasmon Resonance software provides flexibility for users, enabling full control of experimental methods easily transition from collection to analysis to answers.