Example 1. Insulin Like Growth Factor I binding to immobilized Insulin Like Growth Factor Binding Protein 3
Instrument: Reichert SR7000DC Dual Channel SPR Spectrometer
Surface: Planar dithiol mixed self-assembled monolayer presenting –COOH, Catalog Number 13206062
Temperature: 25° C
Autosampler: Reichert/Spark Endurance
Immobilized Species: 950 µRIU Insulin Like Growth Factor Binding Protein 3
Analyte: Insulin Like Growth Factor 1 (IGF1)
flow rate: 50 µL/min
buffer: PBST
3 min 20 sec injections, 3 min 30 sec dissociations
Results:
| ka (M-1 s-1) |
kd (s-1) |
KD, nM |
Surface |
| 6.4e5 |
0.021 |
33.4 |
Planar |
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