Example 2. 4-carboxybenzenesulfonimide binding to immobilized carbonic anhydrase II
Method: Myszka D. G. , et. al. 2003. Journal of Biomolecular Techniques 14: 247-269
Instrument: Reichert SR7000DC Dual Channel SPR Spectrometer
Surface: High Density Polycarboxylate Surface
Temperature: 25° C
Autosampler: Reichert/Spark Endurance
Immobilized Species: 15,171 µRIU Carbonic Anhydrase II
Analyte: CBS
flow rate: 100 µL/min
buffer: 20mM Na2HPO4 - NaH2PO4 and 150mM NaCl at pH 7.4 (PBS)
1 min injections, 3 minute dissociations
| Results: |
ka (M-1 s-1) |
kd (s-1) |
KD, µM |
| SR7000DC |
2.88 e+4 |
0.0298 |
1.03 |
| Lt Reference |
4.0 e+4 |
0.0360 |
0.9 |
Tech Bulletin (PDF 150 KB)
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