Surface Plasmon Resonance (SPR) is most commonly used to determine kinetic rates of reactions (association, dissociation and equilibrium dissociation). In addition, thermodynamic values can also be calculated for the biomolecular interaction being studied. Thermodynamic determinations can add useful additional information to scientific studies about how ligands and proteins interact – beyond basic structural affinity.
Thermodynamic analysis is well suited to Reichert SPR instruments, due to its precise temperature control from 10oC below ambient to 70oC. By running SPR experiments at multiple temperatures, thermodynamic information like entropy and enthalpy can be readily obtained by application of the Van't Hoff equation.
For an example of thermodynamic analysis using the interaction between carbonic anhydrase II and 4-carboxybenzenesulfonamide as an example, see our Application Note on Thermodynamic Analysis.