SPR Concentration Analysis
Surface Plasmon Resonance (SPR) can be used as a very precise method for determining the concentration of a biomolecule. This type of analysis is based on the concept that as the concentration of an analyte increases, the rate of binding to an immobilized binding partner increases. Responses are determined at a time point in the sensorgram of the researcher’s choosing (during either the association or dissociation part of a SPR binding experiment). A calibration curve is generated by plotting responses for standard samples versus their known concentration. If the response time is chosen early in the sensorgram, the plot will be linear. Control samples and samples with unknown concentrations of the analyte are injected and responses are monitored at the same time point as for the standard solutions. The concentration of the analyte in the control or unknown is determined based on the standard calibration curve.