Surface Plasmon Resonance Applications/Protocols

SR7000DC Application Examples

• Insulin Like Growth Factor binding to immobilized Insulin Like Growth Factor Binding Protein 3
• 4-carboxybenzenesulfonamide (MW 200 Daltons) binding to immobilized Carbonic Anhydrase II
• Human Serum Albumin binding to immobilized anti-Human Serum Albumin
• Prostrate Specific Antigen binding to immobilized anti- Prostrate Specific Antigen

Steps of a Typical SPR Experiment:

1. Clean Instrument
2. Immobilize Ligand
3. Inject Analyte (binding partner)
4. Regenerate Surface
5. Analyze Data

Immobilization Strategies (PDF 27.9 kb)