Example 4. Prostrate Specific Antigen binding to immobilized Prostrate Specific Antigen IgG
Method: Katsamba, et. al. 2006. Analytical Biochemistry 352: 208-221
Instrument: Reichert SR7000DC Dual Channel SPR Spectrometer
Surface: Planar dithiol mixed self-assembled monolayer presenting –COOH, Catalog Number 13206062
Autosampler: Reichert/Spark Endurance
Immobilized Species: 2650 uRIU PSA antibody
Analyte: PSA
flow rate: 50 uL/min
buffer 10 mM Phosphate pH 7.4, 139 mM NaCl, 2.7 mM KCl, 0.05% Tween20, and 0.2 mg/mL BSA
3 min injections, 6 minute dissociations
Results:
| Results: |
ka (M-1 s-1) |
kd (s-1) |
KD, nM |
Surface |
| SR7000DC |
1.55 e5 |
1.08 e-4 |
0.7 |
Planar |
| Lt Reference |
4.1 e4 |
4.5 e-5 |
1.1 |
Hydrogel |
Tech Bulletin (PDF 87.8 KB)
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