Example 3. Human Serum Albumin binding to immobilized Anti-Human Serum Albumin IgG
Instrument: Reichert SR7000DC Dual Channel SPR Spectrometer
Surface: Planar dithiol mixed self-assembled monolayer presenting –COOH, Catalog Number 13206062
Autosampler: Reichert/Spark Endurance
Immobilized Species: 1000 µRIU HSA antibody
Analyte: HSA
flow rate: 50 µL/min
buffer 10 mM Phosphate pH 7.4, 139 mM NaCl, 2.7 mM KCl, 0.05% Tween20
6 min injections, 6 minute dissociations
Results:
| ka (M-1 s-1) |
kd (s-1) |
KD, nM |
Surface |
| 4.09e5 |
8.087 e-4 |
1.98 |
Planar |
Tech Bulletin (PDF 96.1 KB)
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